ZRB2176

Anti-MZB1 Antibody, clone 1J15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Marginal zone B- and B1-cell-specific protein;Mesenteric estrogen-dependent adipose 7;MEDA-7;Plasma cell-induced resident endoplasmic reticulum protein;Plasma cell-induced resident ER protein;pERp1;Proapoptotic caspase adapter protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J15, recombinant monoclonal
• description: 1J15 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 20.69 kDa; observed mol wt ~18 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_057543
• UniProt accession no.: Q8WU39
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MZB1(51237)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Marginal zone B- and B1-cell-specific protein (MZB1).

Immunogen

His-tagged, recombinant full-length human Marginal zone B- and B1-cell-specific protein (MZB1) (without the signal peptide).

Application

Quality Control Testing

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MZB1 in Daudi cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MZB1 in Raji, Ramos, PBMC, HEK293T, and HeLa cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MZB1 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MZB1 in Raji cells.

Affinity Binding Assay: A representative lot of this antibody bound recombinant human MZB1 protein with a KD of 1.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Marginal zone B- and B1-cell-specific protein (UniProt: Q8WU39; also known as Mesenteric estrogen-dependent adipose 7, MEDA-7, Plasma cell-induced resident endoplasmic reticulum protein, Plasma cell-induced resident ER protein, pERp1, Proapoptotic caspase adapter protein) is encoded by the MZB1 (also known as MEDA7, PACAP) gene (Gene ID: 51237) in human. MZB1 is a B-cell-specific and endoplasmic reticulum (ER)-localized protein that is implicated in antibody secretion and integrin-mediated cell adhesion. It plays an important role in the humoral immune response by enhancing the interaction of the immunoglobulin (Ig) heavy chain with the chaperone GRP94 and by augmenting the secretion of IgM. It is a part of the ER chaperone complex that contains a large number of molecular chaperones, which associates with unassembled incompletely folded immunoglobulin heavy chains. It associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. It is abundantly expressed in innate-like B cells, including Marginal zone (Mz) B cells of the spleen and peritoneal B1 B cells, as well as in antibody-secreting cells. Deletion of MZB1 results in a reduced humoral response with plasma cells secreting lower levels of IgM. Deletion of MZB1 is shown to impair humoral immune responses and antibody secretion in plasma cells. Five isoforms of MZB1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kapoor, T., et al. (2020). Sci. Rep. 10; Article number: 21626; Rosenbaum, M., et al. (2014). Genes Dev. 28(11); 1165-1178).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable