ZRB1928

Anti-Nibrin/NBS1 Antibody, clone 3L23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Cell cycle regulatory protein p95, NBS, NBS1, Nijmegen breakage syndrome protein 1, hNbs1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3L23, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 84.96 kDa; observed mol wt ~85 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_002476
• UniProt accession no.: O60934
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... NBN(4683)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3L23 is a ZooMAb^® recombinant rabbit monoclonal antibody that detects Nibrin/NBS1. It targets an epitope within the C-terminal half.

Immunogen

His tagged, recombinant fragment corresponding to 335 amino acids from the C-terminal half of human Nibrin/NBS1.

Application

Quality Control Testing

Evaluated by Western Blotting in nuclear extract from HeLa cells.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Nibrin/NBS1 in nuclear extract from HeLa cells.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nibrin/NBS1 in HeLa cells.

Affinity Binding Assay: A representative lot of this antibody bound Nibrin/NBS1 protein with at least one thousand-fold (1,000X) higher than with non-specific GST control.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nibrin/NBS1 in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Nibrin (UniProt: O60934; also known as Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, hNbs1) is encoded by the NBN (also known as NBS, NBS1, P95) gene (Gene ID: 4683) in human. Nibrin consists of multiple functional domains including forkhead-associated (FHA) and BRCA1 C-terminus (BRCT) domains. The protein typically forms a complex called MRN, along with MRE11 and RAD50. Nibrin is primarily involved in the cellular response to DNA damage, particularly in the repair of double-strand breaks through homologous recombination and non-homologous end joining pathways. It acts as a signal transducer by recognizing sites of damage and recruiting other proteins to the site, facilitating DNA repair. Nibrin also plays a role in maintaining telomere integrity and in the activation of cell cycle checkpoints in response to DNA damage. It is primarily localized in the nucleus. Upon DNA damage, it is recruited to the sites of double-strand breaks where it co-localizes with other DNA repair machinery components. Mutations in the NBN gene are directly linked to Nijmegen Breakage Syndrome (NBS), a rare autosomal recessive disorder characterized by microcephaly, immunodeficiency, radiosensitivity, and a predisposition to cancer. Individuals with NBS typically have mutations that lead to truncated forms of nibrin, impairing its ability to effectively participate in DNA repair processes. This deficiency in DNA repair mechanisms significantly contributes to the disease pathogenesis, including developmental abnormalities and cancer predisposition. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable