ZRB1824

Anti-Rab10 Antibody, clone 1G2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1G2, recombinant monoclonal
• description: 1G2 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 22.54 kDa; observed mol wt ~23 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey, canine, bovine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_057215
• UniProt accession no.: P61026
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... RAB10(10890)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G2 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Rab10. It targets an epitope within 23 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of Human Rab10.

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab10 in MCF-7 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab10 in lysates fro A549 cells, Mouse brain tissue, and C6 cells.

Affinity Binding Assay: A representative lot of this antibody bound Rab 10 peptide with a KD of 1.4 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Rab10 in human brain and mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Ras-related protein Rab-10 (UniProt: P61026; also known as Rab10 ) is encoded by the RAB10 gene (Gene ID: 10890) in human. Rab10, a member of the Rab family of small GTPases, is a key regulator of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. It is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. It also plays a specific role in asymmetric protein transport to the plasma membrane. Rab10 localizes to the endoplasmic reticulum (ER) and to dynamic ER-associated structures that track along microtubules and mark the position of new ER tubule growth. Depletion of Rab10 alters ER morphology that results in fewer ER tubules. In the brain, Rab10 is expressed in all cell types. It is activated by phosphorylation at threonine 73 by LRRK2. The phosphorylated Rab10 is reported to colocalize with neurofibrillary tangles in Alzheimer s disease brains. In a cellular model of amyloid precursor protein (APP) metabolism, silencing of RAB10 gene is reported to lead to a significant reduction in Aβ42 and the Aβ42/40 ratio. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tavana, JP., et al. (2019).Clin. Interv. Aging. 14; 73-79; English, AR., and Voeltz, GK. (2013). Nat. Cell Biol. 15(2); 169-178).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable