ZRB1536

Anti-C-Reactive Protein Antibody, clone 1J2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CRP
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J2, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 25.04 kDa; observed mol wt ~30 kDa
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_000558
• UniProt accession no.: P02741
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CRP(1401)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects C-Reactive Protein (CRP). It targets an epitope within 17 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the internal region of human C-Reactive Protein.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat Kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected C-Reactive Protein in Rat kidney tissue lysate.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected C-Reactive Protein in rat kidney tissue sections.

Enzyme Immunoassay (ELISA) Analysis: A Serial of dilutions from a representative lot detected C-Reactive Protein in C-Reactive and GST Proteins.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Rat Kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected C-Reactive Protein in Rat kidney tissue lysate.

Target description

C-reactive protein (UniProt: P02741; also known as CRP) is encoded by the CRP (also known as PTX1) gene (Gene ID: 1401) in human. CRP is an acute-phase protein that is synthesized by hepatocytes in response to pro-inflammatory cytokines during inflammation and infection. It is an evolutionarily conserved major acute-phase protein whose levels may increase over a 1,000-fold in severe inflammatory state. It is synthesized with a signal peptide (aa 1-18) that is subsequently cleaved off to produce the mature protein. Human CRP is a pentameric protein composed of five identical non-covalently bound subunits that bind to phosphocholine (PCh) in a Ca2+-dependent manner and leading to the activation of the complement system via the C1Q complex. Each subunit has one PCh-binding site and each subunit binds two calcium ions. Interleukin-1 and 6, produced in response to injury or infection, are two major triggers for its synthesis. In mature CRP, glutamic acid 81 in the PCh-binding site is reported to interact with the nitrogen atom of choline in PCh and phenylalanine 66 interacts with three methyl groups of choline. The phosphate group of PCh directly coordinates with the two Ca2+ bound to CRP. Under acidic pH conditions, native pentameric CRP can be transformed into a non-native CRP, which exposes a hidden ligand-binding site for non-PCh ligands and enables CRP to bind to immobilized, denatured, and aggregated proteins. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pathak, A., and Agarwal, A. (2019). Front. Immunol. 10; 943; Luan, Y-Y., and Yao, Y-M (2018). Front. Immunol. 9; 1302).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable