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General description
Beacon Designer helps design successful SYBR™ Green, TaqMan™, LNA spiked TaqMan, molecular beacon for both standard and NASBA assays and FRET assays by interpreting BLAST results for specificity and locating oligos avoiding template structures.
SYBR Green Primer Design- Highly specific primers are designed by avoiding significant homologies found by automatically interpreting BLAST search results. Primer efficiency is enhanced by avoiding template secondary structures identified by connecting to the Mfold server.
Dual Labeled Probe Design: Dual-labeled fluorescent TaqMan, molecular beacon or FRET probes can be designed with the help of Beacon Designer. For these assays, you can design new primers and probes or find probes compatible with previously designed SYBR Green assays and save on synthesis costs.
TaqMan probes and Molecular Beacon Multiplex experiments- Multiplex assays are designed by avoiding homologies with all primers and probes in the pool. This prevents competition in multiplex real time PCR reactions and assures high signal strength.
Allelic Discrimination- Design both wild and mutant probes for SNP genotyping assays.
LNA Probe Design- In addition to standard TaqMan probes, Beacon Designer also designs the increasingly popular LNA or Locked Nucleic Acid substituted TaqMan probes to improve real-time PCR assay success.
NASBA Assays- Beacon Designer designs molecular beacon probes for NASBA assays for single template, multiplex or alleleic discrimination.
BLAST Search- Both primers and templates/amplicons can be BLAST searched against genomic databases available at NCBI or against local custom databases. This function helps verify the primer specificity.
Avoid Template Structures- Automatically avoid secondary structures in the template identified by connecting to the Mfold server while designing primers.
Generate Report- Generate an attractively formatted
SYBR Green Primer Design- Highly specific primers are designed by avoiding significant homologies found by automatically interpreting BLAST search results. Primer efficiency is enhanced by avoiding template secondary structures identified by connecting to the Mfold server.
Dual Labeled Probe Design: Dual-labeled fluorescent TaqMan, molecular beacon or FRET probes can be designed with the help of Beacon Designer. For these assays, you can design new primers and probes or find probes compatible with previously designed SYBR Green assays and save on synthesis costs.
TaqMan probes and Molecular Beacon Multiplex experiments- Multiplex assays are designed by avoiding homologies with all primers and probes in the pool. This prevents competition in multiplex real time PCR reactions and assures high signal strength.
Allelic Discrimination- Design both wild and mutant probes for SNP genotyping assays.
LNA Probe Design- In addition to standard TaqMan probes, Beacon Designer also designs the increasingly popular LNA or Locked Nucleic Acid substituted TaqMan probes to improve real-time PCR assay success.
NASBA Assays- Beacon Designer designs molecular beacon probes for NASBA assays for single template, multiplex or alleleic discrimination.
BLAST Search- Both primers and templates/amplicons can be BLAST searched against genomic databases available at NCBI or against local custom databases. This function helps verify the primer specificity.
Avoid Template Structures- Automatically avoid secondary structures in the template identified by connecting to the Mfold server while designing primers.
Generate Report- Generate an attractively formatted
Legal Information
SYBR is a registered trademark of Life Technologies
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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