U5379
Anti-Ubiquitin antibody produced in rabbit
whole antiserum, lyophilized powder
Synonym(s):
Anti-Ubiquitin antibody
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
mol wt
antigen 8.6 kDa
species reactivity
prokaryotes, eukaryotes
technique(s)
immunohistochemistry (frozen sections): suitable
western blot: 1:100 using ubiquitin purified from bovine red blood cells
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... UBB(7314) , UBC(7316)
mouse ... Ubb(22187) , Ubc(22190)
rat ... Ubb(192255) , Ubc(50522)
General description
Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2.
Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes.
Specificity
By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.
Immunogen
bovine red blood cell ubiquitin.
Application
Anti-Ubiquitin antibody produced in rabbit is used in:
- immunostaining in neuropathology
- immunohistochemistry
- western blotting
Biochem/physiol Actions
Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis.
Target description
Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.
Physical form
Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.
Reconstitution
Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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