T7539
Tumor Necrosis Factor-α from mouse
TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
mTNF-α, TNF-α
Sign Into View Organizational & Contract Pricing
All Photos(4)
About This Item
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in E. coli
assay
≥95%
form
powder
potency
0.01-0.5 ng/mL EC50
mol wt
17 kDa
packaging
pkg of 10 μg
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg protein endotoxin
color
white
solubility
water: soluble
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Tnf(21926)
Related Categories
Application
Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
- to activate MS1 endothelium cells
- to induce lipolysis in adipocytes
- to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Unit Definition
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
Physical form
Lyophilized from 10 mM sodium phosphate, pH 7.5
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
SDF-1--CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1--SLIT2 interactions in mice
Glawe JD, et al.
Diabetologia, 56(10), 2222-2230 (2013)
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-induced lipolysis in adipocytes
Yang X, et al.
The Journal of Biological Chemistry, 286(47), 40477-40485 (2011)
Glucocorticoid-independent repression of tumor necrosis factor (TNF) $\alpha$-stimulated interleukin (IL)-6 expression by the glucocorticoid receptor a potential mechanism for protection against an excessive inflammatory response
Verhoog NJD, et al.
The Journal of Biological Chemistry, 286(22), 19297-19310 (2011)
Jing Deng et al.
Molecular and cellular biology, 24(23), 10161-10168 (2004-11-16)
Numerous stressful conditions activate kinases that phosphorylate the alpha subunit of translation initiation factor 2 (eIF2alpha), thus attenuating mRNA translation and activating a gene expression program known as the integrated stress response. It has been noted that conditions associated with
Fen Li et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 40(9), 945-954 (2015-09-27)
To investigate the association of TNF-α-308 gene polymorphism with the risk of RA in Hunan's Han Chinese population. A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNF-α-308 (rs1800629)
Articles
Lipid Induced Insulin Resistance
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service