SRP3086

IL-2 Receptor human

recombinant, expressed in (BTI-Tn-5B1-4) High-5 Insect Cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): CD25 antigen, TAC-antigen, soluble IL-2 receptor
• UNSPSC Code: 12352200

Properties

• biological source: human
• recombinant: expressed in (BTI-Tn-5B1-4) High-5 Insect Cells
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 0.5-15 μg/mL ED₅₀
• mol wt: 31 kDa
• packaging: pkg of 25 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P01589
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL2RA(3559)

Description

General description

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rβ, and IL-2Rγ. The IL-2Ra is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2Ra releases the entire extracellular domain of IL-2Ra thereby generating a 219 amino acid soluble protein called soluble IL-2Ra (sIL-2Ra). The homodimeric form binds IL-2 (KD=10 mM) and facilitates IL-2 signaling. The secreted sIL-2Ra is expressed on leukemia cells, lymphoma cells, newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant human sIL-2R alpha is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. Due to glycosylation, IL-2R alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and Mass Spectrometry.

Biochem/physiol Actions

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Ra, IL-2Rb, and IL-2Rg. Recombinant human sIL-2R alpha is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. Due to glycosylation, IL-2R alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and Mass Spectrometry.

Sequence

ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ

Physical form

Lyophilized from 1x PBS.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable