SRP0101

HDAC 1 from Plasmodium falciparum

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

• Synonym(s): HD1, Histone deacetylase 1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: Plasmodium falciparum
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥40% (SDS-PAGE)
• form: aqueous solution
• mol wt: 55 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: XM_001352091
• UniProt accession no.: Q9XYC7
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: Plasmodium falciparum ... HDAC1(813532)

Description

General description

Plasmodium falciparum HDAC1, GenBank Accession No. XM_001352091, full length with C-terminal HIS6-DDDDK (FLAG) tags, MW = 55 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 50 mM Tris-HCl, pH 8.0, 130 mM NaCl and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable