SAB4503781

Anti-phospho-Smad3 (pSer⁴²⁵) antibody produced in rabbit

affinity isolated antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 48 kDa
• species reactivity: human, rat, mouse
• concentration: ~1 mg/mL
• technique(s): ELISA: 1:40000; immunohistochemistry: 1:50-1:100; western blot: 1:500-1:1000
• NCBI accession no.: P84022
• UniProt accession no.: P84022
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer425)
• Gene Information: human ... SMAD3(4088)

Description

General description

SMAD3 (SMAD family member 3) is located on human chromosome 15q22. Smad 3 belongs to the mothers against Dpp (MAD) related family of proteins.

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425.

Immunogen Range: 376-425

Application

Anti-phospho-Smad3 (pSer425) antibody has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

SMAD (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein Sma) proteins play an important role in the intracellular signalling of transforming growth factor β (TGFβ). Reduction in the phosphorylation level of Smad3 helps autophagy to control the endothelial-mesenchymal transition. In human arterial smooth muscle cells, suppressing siRNA of SMAD3 improves the viability of cells.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable