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SAB4200806

Sigma-Aldrich

Anti-Ezrin antibody, Mouse monoclonal

clone 3C12, purified from hybridoma cell culture

Synonym(s):

Cytovillin, Villin-2, p81

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

3C12, monoclonal

form

buffered aqueous solution

mol wt

~80 kDa

species reactivity

bovine, canine, hamster, kangaroo, human, monkey, rat, mouse

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 1-2 μg/mL using using canine MDCK cells extract
immunofluorescence: 0.5-1  μg/mL using human epidermoid carcinoma A431 cells.

isotype

IgG1

UniProt accession no.

P15311

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Ezr(22350)

General description

Ezrin, also known as cytovillin, p81 or 80K, is a.member of the ERM proteins family composed of Ezrin, Radixin, and Moesin. These proteins are known as actin-binding proteins that link the cell membrane to the actin cytoskeleton, particularly in microvillus-bearing epithelial cells. Ezrin is present in microvilli, microspikes, and membrane ruffles of cells.
The ERM proteins family are involved in the functional expression of membrane proteins on the cell surface, integrate Rho guanosine 5′-triphosphatase (GTPase) signaling regulating cytoskeletal organization and act as protein kinase A (PKA)-anchoring proteins. Therefore, ERM proteins seem to play an important role in the membrane transport of electrolytes by ion channels and transporters.

Immunogen

The carboxy-terminal part of recombinant human ezrin

Application

Monoclonal Anti-Ezrin recognizes an epitope located on the C-terminal region of Ezrin. Reactivity has been observed with human, mouse, hamster, monkey, bovine, canine, rat and rat-kangaroo origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~80kDa), immunofluorescence, Immunohistochemistry and immunoprecipitation.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

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Several cellular factors have been reported to be required for replication of classical swine fever virus (CSFV), a member of the genus Pestivirus within the family Flaviviridae. However, many steps of its replication cycle are still poorly understood. The low-density

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