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S9008

Sigma-Aldrich

Anti-Staphylococcal Enterotoxin B antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Rabbit Anti-SEB

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About This Item

MDL number:
MFCD00082018
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

Staphylococcus aureus

packaging

vial of 2 mL lyophilized antiserum

technique(s)

indirect ELISA: 1:150,000-1:250,000

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Staphylococcal enterotoxin B is a toxic bacterial superantigen. The staphylococcal enterotoxins family have high sequence similarity.

Immunogen

Staphylococcal enterotoxin B from Staphylococcus aureus.

Application

Anti-Staphylococcal Enterotoxin B antibody produced in rabbit has been used:
  • to conjugate with hollow gold nanosphere and as a component of lateral flow immunoassay strip
  • in antibody sandwich immunoassay
  • for coupling with magnetic beads for detection assay

Biochem/physiol Actions

Staphylococcal enterotoxin B (SEB) is a superantigen that binds to class II MHC molecules on antigen presenting cells. It stimulates large populations of T cells resulting in acute toxic shock syndrome. It is resistant to denaturation and causes outbreaks of food poisoning that includes emetic responses, diarrhea and GI inflammation.
Staphylococcal enterotoxin B compromises the immune system by triggering inflammation and cytokine release.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Reconstitution

Reconstitute with 2 ml deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Electrogenerated indium tin oxide-coated glass surface with photosensitive interfaces: Surface analysis
Konry T, et al.
Biosensors And Bioelectronics, 22(9-10), 2230-2236 (2007)
Lily Zhang et al.
Toxins, 5(10), 1859-1871 (2013-10-25)
The functional activity of superantigens is based on capacity of these microbial proteins to bind to both the β-chain of the T cell receptor (TcR) and the major histocompatibility complex (MHC) class II dimer. We have previously shown that a
Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B
Hwang J, et al.
Nanoscale, 8(22), 11418-11425 (2016)
Antje Heit et al.
The Journal of experimental medicine, 214(7), 2139-2152 (2017-06-24)
Germinal center T follicular helper cells (GCTfh) in lymphatic tissue are critical for B cell differentiation and protective antibody induction, but whether GCTfh establish clonal derivatives as circulating memory T cells is less understood. Here, we used markers expressed on
Staphylococcal enterotoxins
Balaban N and Rasooly A
International Journal of Food Microbiology, 61(1), 1-10 (2000)
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