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R8758

Sigma-Aldrich

RPMI-1640 Medium

RPMI-1640 Medium

With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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100 ML
$17.80
500 ML
$35.60
1 L
$56.60
6 X 500 ML
$181.00
6 X 1 L
$308.00
24 X 500 ML
$670.00

$17.80

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100 ML
$17.80
500 ML
$35.60
1 L
$56.60
6 X 500 ML
$181.00
6 X 1 L
$308.00
24 X 500 ML
$670.00

About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

$17.80

In StockDetails

Request a Bulk Order

Product Name

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used for maintaining cell line medium and serves as a medium for different cells.[1][2]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM1129
RNBM1128
RNBM0239
RNBM0243
RNBM0103

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E L Davies et al.
Clinical and experimental immunology, 145(1), 183-189 (2006-06-24)
An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70
Anders Abildgaard et al.
Psychoneuroendocrinology, 79, 40-48 (2017-03-05)
The gut microbiota has recently emerged as an important regulator of brain physiology and behaviour in animals, and ingestion of certain bacteria (probiotics) therefore appear to be a potential treatment for major depressive disorder (MDD). However, some conceptual and mechanistical
Katarzyna A Podyma-Inoue et al.
Cancer genomics & proteomics, 13(6), 443-452 (2016-11-04)
Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments. Subcellular fractionation was used to separate vesicles
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Thaneas Prabakaran et al.
PloS one, 6(9), e25065-e25065 (2011-09-29)
Injury to the glomerular podocyte is a key mechanism in human glomerular disease and podocyte repair is an important therapeutic target. In Fabry disease, podocyte injury is caused by the intracellular accumulation of globotriaosylceramide. This study identifies in the human
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Questions

1–9 of 9 Questions  

  1. I bought this product and realized that it does not contain Hepes. Can I add hepes?

    1 answer

    1. This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.

      Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf

      Helpful?

  2. Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?

    1 answer

    1. The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

  3. buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias

    1 answer

    1. Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf

      Helpful?

  4. Can Product R8758, RPMI-1640 Medium, be frozen?

    1 answer

    1. We do not recommend freezing cell culture media. A precipitate may form upon thawing.  Also, this can cause a loss of amino acids, which may yield low cell growth.

      Helpful?

  5. Does Product R8758, RPMI-1640 Medium, contain HEPES?

    1 answer

    1. There is no HEPES in this formulation of RPMI.  We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

      Helpful?

  6. Does Product R8758, RPMI-1640 Medium, contain phenol red?

    1 answer

    1. This media contains phenol red.  The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

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  7. Does Product R8758, RPMI-1640 Medium, contain glucose?

    1 answer

    1. RPMI contains glucose at 2 g/L.  The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.

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  8. Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?

    1 answer

    1. This formulation of RPMI already contains L-glutamine at 0.3 g/L.  It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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