R6379
Xho I from Xanthomonas holcicola
Restriction Enzyme
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
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grade
Molecular Biology
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-C/TCGAG-3′
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Application
XhoI is a resteriction endonuclease used for molecular biology methods to cut DNA molecules at the recognition sequence 5′-C/TCGAG-3′ to generate fragments with 5′-cohesive ends.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Polydocanol (v/v) at 4 °C
related product
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum.
T R Gingeras et al.
Journal of molecular biology, 118(1), 113-122 (1978-01-05)
H Sesaki et al.
The Journal of cell biology, 152(6), 1123-1134 (2001-03-21)
Membrane fusion plays an important role in controlling the shape, number, and distribution of mitochondria. In the yeast Saccharomyces cerevisiae, the outer membrane protein Fzo1p has been shown to mediate mitochondrial fusion. Using a novel genetic screen, we have isolated
B Haribabu et al.
The Journal of experimental medicine, 192(3), 433-438 (2000-08-10)
Leukotrienes are derived from arachidonic acid and serve as mediators of inflammation and immediate hypersensitivity. Leukotriene B(4) (LTB(4)) and leukotriene C(4) (LTC(4)) act through G protein-coupled receptors LTB(4) receptor (BLTR) and Cys-LTR, respectively. To investigate the physiological role of BLTR
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
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