R1780

RPMI-1640 Medium

With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, sterile liquid, suitable for cell culture, suitable for isotope labeling for cell culture applications

• Synonym(s): RPMI 1640 Cell Culture Medium, Roswell Park Memorial Institute 1640 medium
• MDL number: MFCD00217820
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: RPMI-1640 Medium, With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: 2 g/L; phenol red: no; glucose: 2 g/L (Dextro); L-glutamine: 0.3 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-¹³C₆, ¹⁵N₄ HCl (Product 608033), 0.100 g/L L-Leucine-¹³C₆, ¹⁵N (Product 608068), and 0.100 g/L L-Lysine-¹³C₆, ¹⁵N₂ (Product 608041).

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable