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PPC1010

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Synonym(s):

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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1 ML

$278.00

5 ML

$562.00

$278.00

Available to ship onMarch 17, 2026Details

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C

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Product Name

Protease and Phosphatase Inhibitor Cocktail,

form

DMSO solution

Quality Level

200

packaging

pkg of 1 ml, 5 ml ×

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

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This Item
P1860P8215P8340
Protease and Phosphatase Inhibitor Cocktail

Sigma-Aldrich

PPC1010

Protease and Phosphatase Inhibitor Cocktail

Protease Inhibitor Cocktail for use in tissue culture media, DMSO solution

Sigma-Aldrich

P1860

Protease Inhibitor Cocktail

Protease Inhibitor Cocktail for use with fungal and yeast extracts, DMSO solution

Sigma-Aldrich

P8215

Protease Inhibitor Cocktail

Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts, DMSO solution

Sigma-Aldrich

P8340

Protease Inhibitor Cocktail

form

DMSO solution

form

DMSO solution

form

DMSO solution

form

DMSO solution

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

packaging

pkg of 1 ml, 5 ml ×

packaging

-

packaging

-

packaging

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

-

General description

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction
  • to lyse purified CD4+ T cells
  • to prepare protein extracts from placental tissues and cells

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C

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Certificates of Analysis (COA)

Lot/Batch Number
0000469075
0000469076
0000469075
0000469076
0000201980

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TAGLN2-regulated trophoblast migration, invasion and fusion are impaired in preeclampsia
Wang H, et al.
Frontiers in Cell and Developmental Biology, 10, 810633-810633 (2022)
Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers
Quitterer U, et al.
Cell, S0092-8674(18), 31406-31406 (2018)
Ramaian Santhaseela Anand et al.
Cell stress & chaperones, 25(6), 945-953 (2020-06-24)
Structures of cellular organelles are intertwined with their functions that undergo alterations once the organelles are stressed. Since organelle functions are dependent on each other, an organelle-specific stress possibly influences the structure and function of its associated organelles. In this
miR-433 suppresses tumor progression via Smad2 in non-small cell lung cancer
Li J, et al.
Pathology Research and Practice, 215(10), 152591-152591 (2019)
LKB1-PTEN axis controls Th1 and Th17 cell differentiation via regulating mTORC1
Pandit M, et al.
Journal of Molecular Medicine, 99(8), 1139-1150 (2021)
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Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lisis y extracción de proteínas

Los métodos de lisis celular y extracción de proteínas abarcan diversas técnicas, desde la solubilización con detergentes hasta la disrupción mecánica, lo que respalda las necesidades de investigación.

Data Sheet - PPC1010

Instructions

Global Trade Item Number

SKUGTIN
PPC1010-5ML04061835538416
PPC1010-1ML04061835538409

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