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P7032

Pyruvate Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)

Synonym(s):

PDH, Pig heart pyruvate dehydrogenase

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Size/SKUAvailabilityPrice
10 units

Estimated to ship TODAYfromSAINT LOUIS

$427.00
25 units

Estimated to ship TODAYfromSAINT LOUIS

$770.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
0.1-1.0 units/mg protein (Lowry)
Concentration:
≥5.0 mg/mL

$427.00


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form

buffered aqueous glycerol solution

Quality Segment

specific activity

0.1-1.0 units/mg protein (Lowry)

concentration

≥5.0 mg/mL

impurities

polyethylene glycol, trace

foreign activity

α-ketoglutarate dehydrogenase ≤15%

shipped in

dry ice

storage temp.

−20°C

Application

Pyruvate dehydrogenase contributes to linking the glycolysis metabolic pathway to the citric acid cycle and releases energy via NADH. It is used to study how aerobic training effects pyruvate dehydrogenase . It is also used to study the interaction between pyruvate dehydrogenase kinase and the pyruvate dehydrogenase complex as well as the function and regulation of the pyruvate dehydrogenase complex. Product P7032 is provided in a buffered aqueous glycerol solution and is from porcine heart.
The enzyme has been used in a primary enzyme assay to test the ability of certain compounds to reduce PDK- (pyruvate dehydrogenase kinase) catalyzed PDC (pyruvate dehydrogenase complex) inactivation.

Biochem/physiol Actions

Pyruvate dehydrogenase is involved in the decarboxylation of pyruvate and reductive acetylation of lipoic acid within the pyruvate dehydrogenase complex (PDC). Pyruvate dehydrogenase kinase (PDK) inactivates pyruvate dehydrogenase while pyruvate dehydrogenase phosphatase activates pyruvate dehydrogenase.

Physical form

Supplied as a 50% glycerol solution containing ~9 mg/mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3 TRITON® X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8

Other Notes

One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of Coenzyme A.

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This Item
K1502427211L7525
description

buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)

description

buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)

description

-

description

ammonium sulfate suspension, ≥200 units/mg protein

specific activity

0.1-1.0 units/mg protein (Lowry)

specific activity

0.1-1.0 units/mg protein (Lowry)

specific activity

≥300 units/mg protein

specific activity

≥200 units/mg protein

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized

form

ammonium sulfate suspension

concentration

≥5.0 mg/mL

concentration

-

concentration

-

concentration

-

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

impurities

polyethylene glycol, trace

impurities

-

impurities

≤0.03% GPT, GOT, ≤0.01% PK, MK, ≤0.1% MDH

impurities

-


Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Signal Word

Danger

Hazard Codes

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1



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Questions

  1. Hi I want to know what is the expiration for this product? e.g. 1 year, 2 years,3 years. Thanks, Aviv.

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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