Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

P6407

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Synonym(s):

PDL

Sign Into View Organizational & Contract Pricing

Select a Size

5 MG
$89.60
10 X 5 MG
$658.00

$89.60

Estimated to ship onFebruary 12, 2025

Request a Bulk Order
All Photos(1)

Select a Size

Change View
5 MG
$89.60
10 X 5 MG
$658.00

About This Item

Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
27964-99-4
MDL number:
MFCD00131934
UNSPSC Code:
12352202
PubChem Substance ID:
24898764
NACRES:
NA.75

$89.60

Estimated to ship onFebruary 12, 2025

Request a Bulk Order

Product Name

Poly-D-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

biological source

synthetic (organic)

Quality Level

200

sterility

γ-irradiated

product line

BioReagent
BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 10 X 5 mg
pkg of 5 mg

concentration

0.016—0.032 mmol lysine

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

<10% water (by Karl Fischer)

solubility

H2O: soluble 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Poly-D-lysine hydrobromide has been used:

  • as a coating for plates used in culturing rat glial precursor cells (RGPCS).[1]
  • in coating flasks and plates in culturing tanycytes[2]
  • in preparing the surface of coverslips for facilitating cell attachment[3]
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Features and Benefits

  • Sterilized using gamma (γ) radiation.
  • Sterile Poly-D-Lysine solutions can be stored for three years when stored at 2-8˚C.

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 70,000-150,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000335270
0000354188
0000341162
0000326084
0000338063

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Katherine A Mifflin et al.
Pain, 160(4), 870-881 (2018-12-13)
Multiple sclerosis (MS) is an inflammatory, neurodegenerative autoimmune disease associated with sensory and motor dysfunction. Although estimates vary, ∼50% of patients with MS experience pain during their disease. The mechanisms underlying the development of pain are not fully understood, and
Charles Van der Henst et al.
Nature communications, 9(1), 3460-3460 (2018-08-29)
Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonly found in aquatic habitats. Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, with Acanthamoeba castellanii being one of
Yunze Yang et al.
ACS nano, 12(5), 4186-4193 (2018-03-24)
Action potentials in neurons have been studied traditionally by intracellular electrophysiological recordings and more recently by the fluorescence detection methods. Here we describe a label-free optical imaging method that can measure mechanical motion in single cells with a sub-nanometer detection
Paclitaxel is effective for controlling astrocyte proliferation in vitro: Implications for generating ventral mesencephalic cultures enriched with dopamine neurons
Galvez and Gates MA
Journal of Neuroscience Methods (2020)
Thibaut Brunet et al.
Science (New York, N.Y.), 366(6463), 326-334 (2019-10-19)
Collective cell contractions that generate global tissue deformations are a signature feature of animal movement and morphogenesis. However, the origin of collective contractility in animals remains unclear. While surveying the Caribbean island of Curaçao for choanoflagellates, the closest living relatives
View All Related Papers

Articles

Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

Questions

1–10 of 11 Questions  

  1. 이 가루는 3차 증류수에 녹이면 될까요? 녹인 PDL은 -20에 보관하면 되나요?

    1 answer

    1. This product is tested for solubility in water at 50 mg/mL. The product, in solution, is quite stable when stored at 2-8°C. The freeze/thaw stability of this material has not been investigated. However, the thawing process may cause degradation. The product P4832 is a prepared solution that is stored refrigerated and assigned a 2 year shelf life.

      Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/784/704/p6407pis.pdf

      Helpful?

  2. Comparitively which is better to use with Human embryonic kidney cells, P6407 or P2636?

    1 answer

    1. Comparison testing of these products on Human embryonic kidney cells has not been conducted. In general, P6407 is preferred due to the fact that P2636, provided in L form, is more susceptible to degradation by proteases secreted by cell lines compared to the d-form polymer provided in P6407.

      Helpful?

  3. Is there a standard method of determining the quality of the coating? (Particular focus should be placed on ensuring that the cells adhere as evenly as possible)

    1 answer

    1. As mentioned on the product detail page, this product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high molecular weight versions provide more attachment sites per molecule.

      For more information on using this product for quality coating, please read through the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/784/704/p6407pis.pdf

      Helpful?

  4. How long can I store poly-lysine coated slides or plates?

    1 answer

    1. Plates and/or slides can be stored after drying, protected from dust for up to 1 year.  Inspect the plates/slides to make sure there are no cracks in the coating prior to use.  Each lab should determine the maximum length of time for storage based on the application.

      Helpful?

  5. Do you have a protocol for coating cell cultureware or glass coverslips/slides with Product P6407, Poly-D-Lysine?

    1 answer

    1. The protocol for coating surfaces with poly-D-lysine is the same as for poly-L-lysine.  The optimal conditions must be determined for each cell line and application. In general, the following steps can be used.1. Add 50 ml of sterile tissue culture grade water to 5 mg of poly-D-lysine.2. Aseptically coat culture surface with 0.5-1.0 ml of solution per 25 cm2. Rock gently to ensure even coating of the culture surface.3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.4. Allow to dry at least two hours before introducing cells and medium.

      Helpful?

  6. Does Product P6407, Poly-D-lysine hydrobromide, need to be dialyzed to remove the hydrogen bromide?

    1 answer

    1. The hydrogen bromide will not interfere with the cell growth.  The hydrogen bromide salts are used to make our poly-L-lysine solutions that are cell culture tested.  For these products, there is no interference in cell growth from the bromide.

      Helpful?

  7. Why would I use Product P6407, Poly-D-lysine hydrobromide, instead of poly-L-lysine?

    1 answer

    1. Certain cells can digest poly-L-lysine, in which case the poly-D-lysine should be used.

      Helpful?

  8. How do I determine which molecular weight range of poly-lysine to use?

    1 answer

    1. Poly-lysine is used to coat surfaces to increase the charge present. The lower molecular weight poly-L-lysine (30-70 kD) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kD) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kD.  Please check the literature for information on the molecular weight range used for your cell type.

      Helpful?

  9. How should I store solutions of poly-lysine?

    1 answer

    1. Poly-lysine solutions (0.1 to 1 mg/ml) can be sterile-filtered (0.2 um) and should be stored at 2-8 °C.  Solutions should not be stored frozen as thawing can cause a decrease in the size (molecular weight range) of the polymer.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

1–10 of 11 Questions  

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service