Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
P5493
Phosphate buffered saline
10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology
Synonym(s):
PBS
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About This Item
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Product Name
Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture
grade
BioPerformance Certified
for molecular biology
Quality Level
100
200
sterility
0.2 μm filtered
form
solution
storage condition
(Tightly closed)
technique(s)
cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
impurities
Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)
color
colorless
pH
7.2-7.6 (25 °C)
solubility
water: soluble
cation traces
heavy metals (as Pb): ≤5 ppm
suitability
suitable for
suitable for flow cytometry
suitable for microscopy
application(s)
advanced drug delivery
foreign activity
DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected
SMILES string
OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]
InChI
InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5
InChI key
LOKCTEFSRHRXRJ-UHFFFAOYSA-I
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Application
- as a cell wash solution in human dental pulp stem cells (DPSCs)[1]
- for perfusion studies in dissected brain samples[2]
- as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling[3]
- as wash solution for ovary and sperm during in vitro culture and fertilization[4]
Packaging
Other Notes
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the molecular weight of Product No. P5493 (Phosphate buffered saline)?
1 answer-
Product P5493 is a 10× concentrate phosphate buffered saline solution, certified for bio-performance and suitable for cell culture. This solution comprises two primary ingredients diluted in water:
- Disodium hydrogenorthophosphate with a molecular weight of 141.96 g/mol, identified by CAS number 7558-79-4.
- Sodium chloride with a molecular weight of 58.44 g/mol, identified by CAS number 7647-14-5.Helpful?
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What is the formulation of the 10X PBS, P5493?
1 answer-
The exact formulation for this 10X PBS product remains proprietary. It does not contain calcium, magnesium, or glucose. Per the product page: When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.
Helpful?
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What is the concentration of KCl in 10x PBS? Which phosphate salts are used and what is the concentration of each salt?
1 answer-
Please see the responses to each questions below:
Q: What is the concentration of KCl in 10x PBS?
A: No KCl is used. P5493 contains NaCl.Q: Which phosphate salts are used and what is the concentration of each salt?
A: This is specifically a sodium phosphate buffer, not a mixture of sodium phosphate and potassium phosphate buffer. Unfortunately, the detailed composition remains proprietary.Helpful?
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Does Product P5493, Phosphate buffered saline (PBS), contain calcium and magnesium?
1 answer-
No, it does not contain calcium and magnesium.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the concentration of the 10X stock solution of Product P5493, Phosphate buffered saline (PBS)?
1 answer-
As formulated, the 10X solution is 100 mM phosphate and 1.54 M sodium chloride.
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