P2545

Protein A–Agarose

saline suspension

• Synonym(s): Protein A resin
• UNSPSC Code: 41106500
• NACRES: NA.56
• MDL number: MFCD00165563
• Form: saline suspension
• Biological source: Staphylococcus aureus

Properties

• biological source: Staphylococcus aureus
• Quality Segment: 200
• form: saline suspension
• extent of labeling: ~2 mg per mL
• technique(s): antibody protein purification: suitable
• matrix activation: cyanogen bromide
• matrix attachment: amino
• matrix spacer: 1 atom
• capacity: ≥15 mg/mL binding capacity (human IgG)
• storage temp.: 2-8°C

Description

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Protein A−Agarose has been used in the immunoprecipitation

• of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
• of prolyl hydroxylase domain 2 N27 cells
• of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

Safety Information

• Storage Class: 10 - Combustible liquids
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
• Pictograms: GHS07
• Signal Word: Warning
• Hazard Codes: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1