MAK460

NAD+/NADH Assay Kit

Sufficient for 100 fluorometric tests

• Synonym(s): NAD/NADH Quantitation Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: fluorometric
• relevant disease(s): cancer, Alzheimer′s disease; neurological disorders, Parkinson′s disease; aging/geriatric diseases
• storage temp.: −20°C

Description

General description

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Pyridine nucleotides, including nicotinamide adenine dinucleotide (NAD), are crucial in cellular metabolism, impacting how energy is processed and managed within cells. Monitoring the concentrations of NAD and its reduced form, NADH, is vital for understanding cellular energy dynamics and redox balance. NAD, functioning as a central electron carrier, alternates between NAD+ (oxidized) and NADH (reduced) forms, facilitating numerous metabolic reactions. This transformation is essential not only for redox reactions but also for other metabolic functions, such as ADP-ribosylation, which is crucial for regulating protein functions and DNA repair. Additionally, NAD+ acts as a substrate for sirtuins, which are important for energy metabolism and modulating oxidative stress. .

Application

• Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study.: This study investigates the role of glycolysis in the myometrium of patients with adenomyosis, utilizing the NAD+/NADH Assay Kit to assess metabolic alterations (Huang et al., 2024). 10.1080/09513590.2024.2332411


• Jian-Pi-Yi-Shen formula alleviates renal fibrosis by restoring NAD+ biosynthesis in vivo and in vitro.: This research demonstrates how the Jian-Pi-Yi-Shen formula mitigates renal fibrosis through NAD+ biosynthesis, with the NAD+/NADH Assay Kit playing a crucial role in the biochemical analysis (Gao et al., 2023). 10.18632/aging.205352


• Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cells.: The study explores butyrate′s effects on mitochondrial function and apoptosis in cervical cancer cells, employing the NAD+/NADH Assay Kit to measure mitochondrial activity (Zhang et al., 2023). 10.1186/s12906-023-04043-3


• PM2.5 mediates mouse testis Sertoli TM4 cell damage by reducing cellular NAD().: This article investigates the impact of PM2.5 on Sertoli cells in mouse testes, using the NAD+/NADH Assay Kit to track changes in cellular NAD levels (Xu et al., 2023). 10.1080/15376516.2023.2215862


• Synergistic Effect and Mechanism of Plumbagin with Gentamicin Against Carbapenem-Resistant Klebsiella pneumoniae.: The research highlights the synergistic antibacterial effects of plumbagin and gentamicin, with the NAD+/NADH Assay Kit being used to evaluate the metabolic impact on bacterial cells (Chen et al., 2020). 10.2147/IDR.S265753

The NAD+/NADH Assay Kit may be used for:

• Cancer Research
• Neurodegenerative Research
• Age/Geriatric Related Disease Research

Features and Benefits

Broad Detection Range: Detect NAD and NADH levels accurately across a wide range, from 0.02 to 1 μM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement





Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 10 minute room temperature reaction, reducing complexity and saving valuable time and effort.



Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

The kit is suitable for the determination of NAD and NADH and the evaluation of drug effects on NAD/NADH metabolism in cells, tissue extracts etc.

Principle

The NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8B - Non-combustible corrosive hazardous materials