MAK434

Acetaldehyde Assay Kit

Sufficient for 100 colorimetric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• input: cell culture medium; tissue; serum; urine; food(s); beverage(s); plasma
• application(s): agriculture; cosmetics; environmental; food and beverages
• detection method: colorimetric
• relevant disease(s): gastrointestinal diseases
• storage temp.: −20°C

Description

General description

Acetaldehyde (CH₃CHO) is a commonly found aldehyde in nature and widely used in various industries. It is a metabolic byproduct of ethanol in the liver and is toxic to the human body. However, it is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. Individuals deficient in aldehyde dehydrogenase experience accumulation of acetaldehyde upon alcohol consumption, leading to facial and body flushing known as alcohol flush reaction or “Asian flush syndrome.” Acetaldehyde buildup has also been linked to alcohol-related hangover effects. Despite its classification as a carcinogen, acetaldehyde is naturally present in many foods and beverages such as ripe fruit, coffee, and wine.

Application

The Acetaldehyde Assay Kit may be used for:

• Alcohol Metabolism Research
• Hepatology Research
• Neurological Research

Features and Benefits

• Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent and only a 30 minute assay room temperature reaction, saving you time and effort.
• Convenient and Sensitive: Uses only 20 µL of sample for the determination of acetaldehyde with a detection range of 2 µM to 2mM.
• High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, ensuring efficiency and accuracy.

Suitability

The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).

Principle

The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 12 - Non Combustible Liquids