The OD that represents 5mg/dL bilirubin was determined to be 0.52-0.56 during the development of the kit.
MAK126
Bilirubin Assay Kit
sufficient for 180 colorimetric tests
Synonym(s):
Hematoidin Assay Kit
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1 KIT
$389.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 180 colorimetric tests
detection method
colorimetric
relevant disease(s)
hematological disorder; gastrointestinal diseases
storage temp.
2-8°C
General description
Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.
Application
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
Suitable for the detection of of both total and conjugated bilirubin in serum and plasma samples.
Principle
This assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is a typical absorbance range for the 5 mg/dL standard in the MAK126 Bilirubin Assay Kit?
1 answer-
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Can you please confirm me if these assays works with mouse samples?
1 answer-
All three kits have been used with mouse samples.
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Does this kit work with urine samples?
1 answer-
Kit MAK126 has not been tested on urine samples, so it is not something that can be guaranteed, but it is likely that urine would be okay as long as the concentration falls within the detection range of the kit. Bilirubin does not typically contain urine, so it may not exhibit a response from the kit unless the urine in question comes from a subject with a certain liver condition.
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Can I use the Bilirubin Assay Kit (MK126) to do Bilirubin Assay for mouse?
1 answer-
This Billirubin Assay is not species specific. It has not been tested specifically on mouse serum, however there is no reason to suspect that this kit would not be suitable. Please see the links below to review publications utilizing this product with mouse serum:
https://doi.org/10.3389/fphar.2018.00410
https://doi.org/10.1152/ajpheart.00882.2014
https://doi.org/10.1002/path.5685Helpful?
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Hola. Deseo medir bilirrubina total en una colección de sueros que se han mantenido congelados a -70ºC varios meses. Necesitaría saber si su kit es recomendable para estas muestras y la cantidad mínima de suero (humano) que se puede usar por reacción. Gcs
1 answer-
Measurement of Total and Direct Bilirubin requires a total of 150 microliters of sample (50 microliters each for Total, Direct, and Sample Blank). Serum (frozen or fresh) is a suitable sample for use with the kit.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/564/mak126bul.pdf
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