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L9384

Sigma-Aldrich

L-Leucine 3-carboxy-4-hydroxyanilide hydrochloride

suitable for ligand binding assays

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About This Item

Empirical Formula (Hill Notation):
C13H18N2O4 · HCl
CAS Number:
73801-31-7
Molecular Weight:
302.75
MDL number:
MFCD00058075
UNSPSC Code:
12352202
PubChem Substance ID:
24896522

product name

L-Leucine 3-carboxy-4-hydroxyanilide hydrochloride,

technique(s)

ligand binding assay: suitable

storage temp.

2-8°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc(O)c(c1)C(O)=O

InChI

1S/C13H18N2O4.ClH/c1-7(2)5-10(14)12(17)15-8-3-4-11(16)9(6-8)13(18)19;/h3-4,6-7,10,16H,5,14H2,1-2H3,(H,15,17)(H,18,19);1H/t10-;/m0./s1

InChI key

BSVJWFHAORBGDY-PPHPATTJSA-N

Application

L-Leucine 3-carboxy-4-hydroxyanilide may be used as a substrate for determining the activity of microsomal aminopeptidase in serum.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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M Shimamoto et al.
Clinical chemistry, 31(10), 1636-1639 (1985-10-01)
We describe a colorimetric method for assay of microsomal aminopeptidase (EC 3.4.11.2) activity in serum. We use a new substrate, L-leucyl-3-carboxy-4-hydroxyanilide, p-xylenol as coupler, and sodium metaperiodate as oxidizing reagent. The colored substance formed by the oxidative condensation between p-xylenol

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