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L8257

Sigma-Aldrich

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥80 units/mg protein

Synonym(s):

Donor:hydrogen peroxide oxidoreductase, Peroxidase

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About This Item

CAS Number:
9003-99-0
Enzyme Commission number:
1.11.1.7 (BRENDA, IUBMB)
EC Number:
232-668-6
MDL number:
MFCD00131484
UNSPSC Code:
12352204

form

lyophilized powder (essentially salt-free)

specific activity

≥80 units/mg protein

absorbance ratio

A412/280 nm 0.88-0.95

UniProt accession no.

P80025

storage temp.

−20°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Gene Information

cow ... LPO(280844)

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Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Unit Definition

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Analysis Note

Protein determined by Lowry method.

Other Notes

Using 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt as substrate, two or three times the activity is observed.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A M Lew et al.
Proceedings of the National Academy of Sciences of the United States of America, 83(16), 6084-6088 (1986-08-01)
In addition to mRNA encoding the canonical form of the murine class I antigen H-2Kb (348 amino acid residues), mRNA that would encode a shortened form of H-2Kb (missing 9 amino acids from the C-terminus) has been identified in C57BL/6
Tracing with radiolabeled neurotrophins.
C S von Bartheld
Methods in molecular biology (Clifton, N.J.), 169, 195-216 (2001-01-06)
Lior Cohen et al.
The Journal of biological chemistry, 283(22), 15169-15176 (2008-03-15)
The bioactive surface of scorpion beta-toxins that interact with receptor site-4 at voltage-gated sodium channels is constituted of residues of the conserved betaalphabetabeta core and the C-tail. In an attempt to evaluate the extent by which residues of the toxin
Karolina Haberska et al.
Talanta, 76(5), 1159-1164 (2008-09-02)
Lactoperoxidase (LPO) is an enzyme, which is used as an antimicrobial agent in a number of applications, e.g., food technology. In the majority of applications LPO is added to a homogeneous product phase or immobilised on product surface. In the
José Serrano et al.
Biochemical and biophysical research communications, 388(2), 443-449 (2009-08-15)
Oxidation of Amplex Red by hydrogen peroxide in the presence of horseradish peroxidase (HRP) gives rise to an intensely colour product, resorufin. This reaction has been frequently employed for measurements based on enzyme-coupled reactions that detect hydrogen peroxide as a
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