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L3794

Sigma-Aldrich

LOK (1-348), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

PRO2729, STK10

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

100

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

2-4 nmol/min·mg

mol wt

~65 kDa

UniProt accession no.

O94804

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... STK10(6793)

General description

LOK (lymphocyte-oriented kinase) belongs to the Ste20 (sterile 20) family of serine/threonine kinases. It is expressed in lymphocytes, brain, colon, thymus, kidney, liver, small intestine and lung. LOK is commonly referred to as STK10 (serine/threonine protein kinase 10).

Biochem/physiol Actions

Mutations in LOK (lymphocyte-oriented kinase) are linked with the anti-apoptotic effects leading to carcinogenesis. It functions as an ERM (ezrin-radixin-moesin) kinase and thereby regulates cytoskeletal organization and lymphocyte migration. It can phosphorylate PLK1 (polo-like kinase 1).

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kazutaka Fukumura et al.
Oncology reports, 30(4), 1542-1548 (2013-07-12)
Peripheral T-cell lymphoma (PTCL) is an aggressive lymphoma with a 5-year overall survival rate of <30%. To identify carcinogenesis-related genes in PTCL, we conducted high-throughput resequencing of target-captured cDNA in a PTCL specimen, revealing a total of 19 missense mutations
Sarah A Walter et al.
The Journal of biological chemistry, 278(20), 18221-18228 (2003-03-18)
The Ste20 family of serine/threonine kinases plays an important role in numerous cellular functions such as growth, apoptosis, and morphogenesis. We have identified a previously cloned but uncharacterized family member termed Stk10, which is a human homolog of murine Lok
Natalya V Belkina et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(12), 4707-4712 (2009-03-04)
ERM (ezrin-radixin-moesin) proteins mediate linkage of actin cytoskeleton to plasma membrane in many cells. ERM activity is regulated in part by phosphorylation at a C-terminal threonine, but the identity of ERM kinases is unknown in lymphocytes and incompletely defined in
Li Tao et al.
The Biochemical journal, 363(Pt 1), 175-182 (2002-03-21)
T-cell activation requires signals from both the T-cell receptor (TcR) and other co-stimulatory molecules such as CD28. TcR- and CD28-mediated signals are integrated during T-cell activation resulting in the expression of cytokine genes such as interleukin-2 (IL-2). An enhancer element

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