I8523

Interleukin-12 from mouse

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(s): IL-12
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00212975

Properties

• biological source: mouse
• Quality Segment: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.01-.1 ng/mL ED₅₀
• mol wt: glycoprotein 70 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P43431, P43432
• storage temp.: −20°C
• Gene Information: mouse ... Il12a(16159), Il12b(16160)

Description

Application

Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii.[1] It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.[2]

Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)