I7642
Anti-Interleukin-12 antibody produced in goat
IgG fraction of antiserum
Synonym(s):
Anti-IL-12
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About This Item
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biological source
goat
Quality Level
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
technique(s)
neutralization: suitable
western blot: 1-2 μg/mL
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Il12a(16159)
General description
Interleukin-12 (IL-12) is an important cytokine that links innate and adaptive immunity. It plays a role in the regulation of Th1-associated cellular immunity to foreign pathogens, and humoral immunity to antigens that are both T cell-independent and T cell-dependent. IL-12 is primarily produced by innate immune cells, dendritic cells, epithelial cells and macrophages. All forms of IL-12 bind to the IL-12R which includes IL-12RbI and IL-12RbII subunits expressed on T cells and natural cells. IL-12 stimulates the production of IFN-γ and TNF-α, subsequent production of nitric oxide that leads to pathogen destruction. Reports suggest the involvement of JAK/STAT pathway components in mediating these effects. The effects of IL-12 have been implicated in bacterial, fungal and viral infections. Due to its role in inducing matrix metalloproteases, it also has important role in the etiology of chronic B-cell malignancies and lung adenocarcinomas
Anti-Interleukin-12 antibody recognizes mouse IL-12. It shows < 10% cross-reactivity with recombinant human IL-12 and < 5% cross-reactivity with recombinant porcine IL-12.
Anti-Interleukin-12 antibody recognizes mouse IL-12. It shows < 10% cross-reactivity with recombinant human IL-12 and < 5% cross-reactivity with recombinant porcine IL-12.
Specificity
The antibody will neutralize the biological activity of recombinant mouse IL-12. Based on ELISA, the antibody shows ~10% cross-reactivity with recombinant human IL-12 and <5% cross-reactivity with recombinant porcine IL-12.
Immunogen
purified, insect cell line Sf21-derived, recombinant mouse IL-12.
Application
Anti-Interleukin-12 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is also suitable for neutralization reactions. It has been used to neutralize IL-12 in the extracts of murine splenocytes.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Preparation Note
Purified using protein G.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ludwig Deml et al.
Infection and immunity, 73(8), 4732-4742 (2005-07-26)
Predominant T-helper 1 (Th1) responses with increased gamma interferon (IFN-gamma) levels have been proposed to play an important role in Helicobacter pylori-induced gastritis and peptic ulceration. However, bacterial factors contributing to the initiation of Th1 polarization of H. pylori-specific immune
IL-12 and Related Cytokines: Function and Regulatory Implications in Candida albicans Infection
Ashman RB et al
Clinical & Developmental Immunology, doi: 10-doi: 10 (2011)
Anne O'Garra et al.
Nature immunology, 10(9), 929-932 (2009-08-21)
The authors recount their discovery of how pathogen-induced interleukin 12 production leads to T(H)1 T cell polarization. Simultaneously they discovered the suppressive cytokine interleukin 10 inhibits antigen-presenting cells, thus regulating development of T(H)1 cells.
Dennis W Metzger
Cytokine, 52(1-2), 102-107 (2010-07-24)
Interleukin (IL)-12 is a pivotal cytokine that strongly stimulates Th1-associated cellular immunity. It is now recognized that IL-12 also activates humoral immunity to both T-dependent and T-independent antigens. This has let to considerable interest in exploiting IL-12 as a vaccine
David J Klinke
Molecular cancer, 9, 242-242 (2010-09-17)
Monoclonal antibodies represent some of the most promising molecular targeted immunotherapies. However, understanding mechanisms by which tumors evade elimination by the immune system of the host presents a significant challenge for developing effective cancer immunotherapies. The interaction of cancer cells
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