HPA019000
Anti-PHLDA1 antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-Apoptosis-associated nuclear protein, Anti-PQ-rich protein, Anti-Pleckstrin homology-like domain family A member 1, Anti-Proline- and glutamine-rich protein, Anti-Proline- and histidine-rich protein, Anti-T-cell death-associated gene 51 protein
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
orthogonal RNAseq
orthogonal RNAseq
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry: 1:50- 1:200
western blot: 0.4 μg/mL
immunogen sequence
RMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVE
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PHLDA1(22822)
General description
The gene PHLDA1 (pleckstrin homology-like domain family A member 1) is mapped to human chromosome 12q15. It belongs to pleckstrin homology-related domain family. PHLDA1 is widely expressed. The protein is present in the cytoplasm and nucleolus. PHLDA1 is also referred as TDAG51 (T-cell death-associated gene 51).
Immunogen
Pleckstrin homology-like domain family A member 1 recombinant protein epitope signature tag (PrEST)
application
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.
Biochem/physiol Actions
In activated T lymphocytes, PHLDA1 (pleckstrin homology-like domain family A member 1) works with T-cell receptor to inhibit protein biosynthesis. It is also responsible for differentiation of trichoepithelioma from basal cell carcinomas (BCCs). PHLDA1 is down-regulated in primary, and metastatic melanomas and breast cancer. On the other hand, it is involved with migration and proliferation in colon cancer cells. PHLDA1 is also involved in development of atherosclerosis in presence of hyperhomocysteinemia.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Linkage
Corresponding Antigen APREST74199
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Maxime Battistella et al.
The American Journal of dermatopathology, 36(8), 643-650 (2013-05-31)
Granular-cell or clear-cell basal cell carcinomas (BCCs), and clear-cell trichoblastomas have rarely been reported in the literature. PHLDA1 is a follicular stem cell marker, the expression of which has been reported to differentiate trichoepithelioma from BCCs. We wondered whether (1)
T Hinz et al.
Cellular signalling, 13(5), 345-352 (2001-05-23)
The T cell death associated gene 51 (TDAG51) was shown to be required for T cell receptor (TCR)-dependent induction of Fas/Apo1/CD95 expression in a murine T cell hybridoma. Despite the absence of a nuclear localization sequence and a nucleic acid
Assignment of the human PHLDA1 gene to chromosome 12q15 by radiation hybrid mapping.
M D Kuske et al.
Cytogenetics and cell genetics, 89(1-2), 1-1 (2000-07-15)
Anuratha Sakthianandeswaren et al.
Cancer research, 71(10), 3709-3719 (2011-05-12)
Studies employing mouse models have identified crypt base and position +4 cells as strong candidates for intestinal epithelial stem cells. Equivalent cell populations are thought to exist in the human intestine; however robust and specific protein markers are lacking. Here
Emmanuel O Johnson et al.
Journal of cell science, 124(Pt 16), 2711-2722 (2011-08-03)
Aurora A kinase is overexpressed in the majority of breast carcinomas. A chemical genetic approach was used to identify the malignant targets of Aurora A, which revealed pleckstrin-homology-like domain protein PHLDA1 as an Aurora A substrate. PHLDA1 downregulation is a
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