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Key Documents

HCLALKLU

Sigma-Aldrich

ALK-Lung Analyte Control

Cell line controls for immunohistochemistry and in situ hybridization

Synonym(s):

EML4-ALK Analyte Control

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.47

biological source

human cell culture

packaging

pkg of 1 ea cell microarray block (HCL009)
pkg of 2 slides (HCL007)
pkg of 5 slides (HCL008)

manufacturer/tradename

Histocyte

storage temp.

2-8°C

General description

The ALK-Lung Analyte Control has two cell line cores which demonstrate positive and negative expression of EML4-ALK associated lung cancer.
HistoCyte Laboratories control slides and blocks are made from cultured human cell lines to provide the required range of expression of a specific biomarker. The cell lines are processed using proprietary methods that allow for the creation of a high density embedded cell block with cores that retain their cellular morphology. Available as pre-cut unstained slides or as cell microarray blocks for sectioning.

Application

Ideal for use as a same slide control for immunohistochemistry (IHC) and in situ hybridization (ISH).
The ALK-Lung Analyte Control histology slides have two cell line cores providing positive and negative expression of EML4-ALK. Analyte controls are placed at one end of slide, allowing sample application on same slide for valid comparison.
The ALK-Lung Analyte Control cell microarray block contains two cell line cores that are 2 mm in diameter and between 3.0-3.5 mm in length. The cell microarray block may yield over 300 sections depending on thickness.

Preparation Note

Cell microarray blocks are fixed in 10% neutral buffered formalin and embedded in paraffin wax. Pre-cut unstained slides are prepared from 4 μm thick sections, mounted on positively charged slides, and dried at 37 °C overnight.

Other Notes

For certificates of analysis, contact info@histocyte.com.
ALK-Lung Analyte Control brochure is available from HistoCyte Laboratories.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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