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H7787
HIS-Select® Spin Columns
Synonym(s):
His-Tag Spin Columns
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10 EA
$215.00
50 EA
$610.74
$215.00
Estimated to ship onApril 11, 2025
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10 EA
$215.00
50 EA
$610.74
About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
$215.00
Estimated to ship onApril 11, 2025
Recommended Products
shelf life
2 yr at 2‑8 °C
Quality Level
pore size
100 nm pore size
capacity
>0.5 mg/column
storage temp.
2-8°C
General description
Kit contains 3 collection tubes for every spin column.
Application
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.
Features and Benefits
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the Department of Transportation shipping information for this product?
1 answer-
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Can HIS-Select® Spin Column, Product H7787, be re-used?
1 answer-
We do not recommend re-using the columns. Each column is intended for one time use.
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What is the binding capacity of the HIS-Select® Spin Column, Product H7787?
1 answer-
The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.
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