GE29-0005-93

His Spintrap^™ TALON^®

Cytiva 29-0005-93

• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• ligand: TALON^® Superflow Cobalt-IMAC
• manufacturer/tradename: Cytiva 29-0005-93
• packaging: pkg of 50 x 100 μL
• bed volume: 100 μL
• matrix: 6% cross-linked agarose
• particle size: 60-160 μm
• cleaning: 2-14(Co²⁺-stripped medium)
• working range: 3-12(Co2+-stripped medium)
• capacity: ≤1 mg binding capacity(histidine-tagged); ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
• storage temp.: 2-8°C

Description

General description

His SpinTrap^™ Talon^® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Application

His SpinTrap^™ Talon^® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap^™ Talon^® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon^® Superflow medium is negligible.

Features and Benefits

• Suitable for purification of multiple samples in parallel, such as screening.
• Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon^® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• With a microcentrifuge, one run takes about 10 minutes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SpinTrap is a trademark of Cytiva

Talon is a registered trademark of Clontech

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids