GE17-0851-01

Disposable PD 10 Desalting Columns

Cytiva 17-0851-01, pack of 30 pieces

• Synonym(s): Disposable desalting columns, PD-10 columns
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• feature: wetted part: no
• packaging: pack of 30 pieces
• manufacturer/tradename: Cytiva 17-0851-01
• storage condition: dry media
• bed size: 14.5 mm × 50 mm
• bed volume: 8.3 mL
• column I.D.: 15 mm
• matrix: cross-linked dextran
• particle size: >50 μm (dry); 85-260 μm
• cleaning: 2-13
• working range: 2-13
• suitability: suitable for bioprocess medium

Description

General description

Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.

The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.

Features and Benefits

• Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex^® G-25 Medium.
• Flexible protocols allow runs by gravity flow or centrifugation.
• Easy to use and equilibrate; apply sample; elute.
• Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
• Desalt multiple samples in parallel with a desalting capacity >90%.
• Recovery in the range 70 to >95%.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)

Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P280 - P261 - P272 - P302 + P352 - P333 + P313 - P501
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable