GE17-0034-02
Sephadex® G-25 Coarse
Cytiva 17-0034-02, pack of 500 g
About This Item
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packaging
pack of 500 g
manufacturer/tradename
Cytiva 17-0034-02
storage condition
dry media
matrix
cross-linked dextran
particle size
>100 μm (dry)
cleaning in place
2-13
working range
2-13
suitability
suitable for bioprocess medium
General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).
Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Excellent recovery and minimum sample dilution
- Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
- BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications
Storage and Stability
Analysis Note
Legal Information
Storage Class
13 - Non Combustible Solids
Certificates of Analysis (COA)
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Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
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Protocols
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to prepare samples for purification with affinity chromatography.
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