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G6378

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

Synonym(s):

G-6-P-DH, Zwischenferment

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About This Item

CAS Number:
9001-40-5
Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
EC Number:
232-602-6
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type XV

Quality Level

300

form

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

mol wt

128 kDa

purified by

crystallization

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

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General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight

Preparation Note

Prepared from G7877

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
María J Rodríguez-López et al.
Insects, 11(9) (2020-09-13)
A combination of biological control and host plant resistance would be desirable for optimally controlling the greenhouse whitefly, Trialeurodes vaporariorum in tomato crops. Whitefly settlement preference, oviposition, and survivorship were evaluated on ABL 10-4 and 'Moneymaker', two nearly-isogenic tomato lines
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Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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