G0407
Granulocyte colony-stimulating factor human
G-CSF, recombinant, expressed in E. coli, suitable for cell culture
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About This Item
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biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.01-0.1 ng/mL
quality
endotoxin tested
mol wt
18.8 kDa (175 amino acids including N-terminal methionine)
packaging
pkg of 5 and 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg (LAL test)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CSF3(1440)
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General description
Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.
Application
Granulocyte colony-stimulating factor human has been used:
- in culturing human neutrophils
- as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells
- to test its protective effects chronic liver damage model
Biochem/physiol Actions
Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Physical form
Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF
Analysis Note
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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The present study aimed at investigating the beneficial effects of co-administering granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) in a model of chronic liver injury induced by thioacetamide (TAA). Biochemical and histopathology- cal examinations were performed on serum
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Articles
Read article on hematopoietic cytokines and hematopoiesis
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