F8192

Fetal Bovine Serum

Name: Fetal Bovine Serum
Brand: SIGMA

Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

HI FBS, HI sera, HI serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

MDL number:

MFCD00132239

Biological source:

bovine fetus

Origin:

Australia origin

Sterility:

sterile-filtered

Shipped in:

dry ice

Key Documents

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Related Categories

Bovine Serum Albumin

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Fetal Bovine Serum (FBS)

Molecular Biology & Functional Genomics

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1 of 4

This Item	F8067	12006C	12103C
Sigma-Aldrich F8192 Fetal Bovine Serum	Sigma-Aldrich F8067 Fetal Bovine Serum	Sigma-Aldrich 12006C Fetal Bovine Serum	Sigma-Aldrich 12103C Fetal Bovine Serum
biological source bovine fetus	biological source bovine fetus	biological source bovine fetus	biological source bovine fetus
technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
origin Australia origin	origin New Zealand origin	origin Australia origin	origin USA origin
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL	composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL	composition Hemoglobin, ≤25 mg/dL	composition hemoglobin, ≤20 mg/dL
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

Endotoxin and hemoglobin tested

Tested for the presence of bacteria, virus, and mycoplasma
Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

16L577H1

16L571

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Development of Novel antimiRzymes for Targeted Inhibition of miR-21 Expression in Solid Cancer Cells.

Leon M Larcher et al.

Molecules (Basel, Switzerland), 24(13) (2019-07-10)

MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the regulation of gene expression. Previous reports showed an over-expression of miRNA-21 (miR-21) in various cancer cells, and its up-regulation is closely related to cancer initiation, proliferation and metastasis. In

Identification and evaluation of hub mRNAs and long non-coding RNAs in neutrophils during sepsis.

Jiamin Huang et al.

Inflammation research : official journal of the European Histamine Research Society ... [et al.], 69(3), 321-330 (2020-02-07)

To reveal the systematic response of neutrophils to sepsis and to study the hub lncRNAs in sepsis. Neutrophils taken from the femur and tibia of male C57 BL/6 mice were used in this study. And neutrophils were treated for 0 h

Gut Microenvironment and Bacterial Invasion in Paediatric Inflammatory Bowel Diseases.

Deenaz Zaidi et al.

Journal of pediatric gastroenterology and nutrition, 71(5), 624-632 (2020-10-24)

Host-microbial relationship is disrupted in inflammatory bowel diseases (IBD). We hypothesized that altered gut luminal microenvironment can impact microbial virulence in IBD, leading to disruption of homeostasis and disease. We investigated the relationship between gut microenvironment and microbial virulence. Intestinal

Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli.

Hyun Bong Park et al.

Nature microbiology, 5(11), 1319-1329 (2020-07-29)

Escherichia coli is an important model organism in microbiology and a prominent member of the human microbiota1. Environmental isolates readily colonize the gastrointestinal tract of humans and other animals, and they can serve diverse probiotic, commensal and pathogenic roles in

Codon-Optimization of Wild-Type Adeno-Associated Virus Capsid Sequences Enhances DNA Family Shuffling while Conserving Functionality.

Marti Cabanes-Creus et al.

Molecular therapy. Methods & clinical development, 12, 71-84 (2018-12-12)

Adeno-associated virus (AAV) vectors have become one of the most widely used gene transfer tools in human gene therapy. Considerable effort is currently being focused on AAV capsid engineering strategies with the aim of developing novel variants with enhanced tropism

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Global Trade Item Number

SKU	GTIN
F8192-100ML	04061833620380
F8192-1L	04061838113702
F8192-500ML	04061833620403
F8192-50ML	04061838113719

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