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D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Synonym(s):

3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

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500 mg
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$31.50
1 g
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$56.60
5 g
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$139.00
10 g
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$257.00
25 g
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$516.00
100 g
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$1,990.00
$1,691.50

About This Item

Linear Formula:
[-SC6H3(NO2)CO2H]2
CAS Number:
69-78-3
Molecular Weight:
396.35
UNSPSC Code:
12352106
NACRES:
NA.26
PubChem Substance ID:
24894189
EC Number:
200-714-4
Beilstein/REAXYS Number:
1896821
MDL number:
MFCD00007140
Assay:
≥98%
Form:
powder

$31.50

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product line

BioReagent

Quality Segment

200

assay

≥98%

form

powder

mp

240-245 °C (dec.) (lit.)

suitability

suitable for determination of sulfhydryl groups

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent.[1] It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH.[2] Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.[3]

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues.[2] It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

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This Item
D218200322123158194
5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups

Sigma-Aldrich

D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid) ReagentPlus®, 99%

Sigma-Aldrich

D218200

5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithio-bis-(2-nitrobenzoic Acid) Sulfhydryl reagent used to characterize reactive SH groups.

Sigma-Aldrich

322123

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

2,2′-Dithiobis(5-nitropyridine) 96%

Sigma-Aldrich

158194

2,2′-Dithiobis(5-nitropyridine)

assay

≥98%

assay

99%

assay

≥98% (titration)

assay

96%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

form

powder

form

powder

form

solid

form

solid

mp

240-245 °C (dec.) (lit.)

mp

240-245 °C (dec.) (lit.)

mp

-

mp

155-157 °C (lit.)

product line

BioReagent

product line

ReagentPlus®

product line

-

product line

-

suitability

suitable for determination of sulfhydryl groups

suitability

-

suitability

-

suitability

-

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000562983
0000562981
0000562982
0000545626
0000545627

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Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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Product Information Sheet - D8130

Product Information Sheet

Spectra - ATR-IR
View All Related Content
Venkaiah Betapudi et al.
Science translational medicine, 12(527) (2020-01-24)
Chemical warfare nerve agents are organophosphorus chemical compounds that induce cholinergic crisis, leaving little or no time for medical intervention to prevent death. The current chemical treatment regimen may prevent death but does not prevent postexposure complications such as brain
Reassessment of Ellman's reagent.
P W Riddles et al.
Methods in enzymology, 91, 49-60 (1983-01-01)
Christian K Riener et al.
Analytical and bioanalytical chemistry, 373(4-5), 266-276 (2002-07-12)
Since its introduction in 1959, Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay
View All Related Papers

Global Trade Item Number

SKUGTIN
D8130-150MG04061833412954
D8130-10G04061835544141
D8130-50MG04061833690161
D8130-4MG04061833690154
D8130-100G04061835544134
D8130-500MG04061835544172
D8130-8MG04061833460979
D8130-1G04061835544158
D8130-5G04061835544189
D8130-25G04061835544165

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. To dissolve DTNB which alcohol should use?

    1 answer

    1. This product is tested for solubility in Ethanol at 10 mg/mL. Solutions may also be prepared at 5mM in 100 mM sodium or potassium phosphate buffer, pH 7.2, containing 0.1 mM EDTA. Please see the link below to review the entire product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/707/d8130pis.pdf

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  4. what is the storage temperature for this chemical

    1 answer

    1. This product can be stored at room temperature.

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