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D2916

Lambda DNA Mixed Digest

for DNA electrophoresis

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20 μG

$67.90

0.1 MG

$260.00

$67.90

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About This Item

NACRES:
NA.25
UNSPSC Code:
12352200

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Quality Level

200, 100

form

buffered aqueous solution

usage

100 uses (0.1 mg size), 20 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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Lambda DNA Mixed Digest for DNA electrophoresis

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Lambda DNA Mixed Digest

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Lambda DNA Hind III Digest for DNA electrophoresis

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Lambda DNA Hind III Digest

DirectLoad™ 50 bp DNA Step Ladder ready-to-use marker for DNA electrophoresis

Sigma-Aldrich

D3812

DirectLoad™ 50 bp DNA Step Ladder

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

form

buffered aqueous solution

form

liquid

form

liquid

form

(liquid)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100, 200

Quality Level

100, 200

Quality Level

200

Quality Level

200

usage

100 uses (0.1 mg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

usage

sufficient for 100 loads

General description

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Preparation Note

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.4%.
Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000514451
0000514451
SLCF2150
SLCC8909
SLBX9607

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A rapid (100 min) method for isolating high yield and quality DNA from leaves, roots and coleoptile of wheat (Triticum aestivum L.) suitable for apoptotic and other molecular studie
Hameed
International Journal of Agriculture and Biology, 383-387 (2004)

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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