CWDS-APC
ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye
for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)
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About This Item
UNSPSC Code:
41116015
NACRES:
NA.41
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product line
ColorWheel®
Quality Level
form
lyophilized
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
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technique(s)
flow cytometry: suitable
fluorescence
λex 651 nm; λem 660 nm
compatibility
for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
General description
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
Allophycocyanin (APC) belongs to a family of phycobiliproteins and is well studied as a fluorescent reagent for flow cytometric analysis. It is isolated from a Spirulina species, a type of blue-green alga and is composed of α and β subunits. The heterodimers of these subunits can assemble as trimer or hexamer. APC displays high absorptivity and quantum efficiency. It has an excitation max at 651 nm and a emission max at 660 nm with extinction coefficient of 700,000 cm-1 M-1. It can absorb and emit light with low energy losses to quenching. APC can be easily conjugated to monoclonal and polyclonal antibodies for use in fluorescence-based detection methods, such as flow cytometry and FACS analysis. (Ref.: Sonani, RR., et al. (2015). PLoS One. 10(4): e0124580; Jung, TM., and Daily MO. (1989). J. Immunol. Methods. 121(1); 9-18).
Allophycocyanin (APC) belongs to a family of phycobiliproteins and is well studied as a fluorescent reagent for flow cytometric analysis. It is isolated from a Spirulina species, a type of blue-green alga and is composed of α and β subunits. The heterodimers of these subunits can assemble as trimer or hexamer. APC displays high absorptivity and quantum efficiency. It has an excitation max at 651 nm and a emission max at 660 nm with extinction coefficient of 700,000 cm-1 M-1. It can absorb and emit light with low energy losses to quenching. APC can be easily conjugated to monoclonal and polyclonal antibodies for use in fluorescence-based detection methods, such as flow cytometry and FACS analysis. (Ref.: Sonani, RR., et al. (2015). PLoS One. 10(4): e0124580; Jung, TM., and Daily MO. (1989). J. Immunol. Methods. 121(1); 9-18).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Application
Flow Cytometry: Excitation: 651 nm, Emission: 660 nm
Compatibility
Requires ColorWheel® Antibody (Sold Separately)
Physical form
CWDS-APC-25UL Set Components:
CWD-APC-25UL: ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL: ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
CWD-APC-25UL: ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL: ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
Reconstitution
Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
Legal Information
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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