CWA-1061

Anti-Mouse CD62L (MEL-14) Colorwheel^® Dye-Ready mAb

for use with ColorWheel^® Dyes (Required, sold separately)

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MEL-14, monoclonal
• product line: ColorWheel^®
• form: lyophilized
• mol wt: calculated mol wt 42.29 kDa
• species reactivity: mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable
• isotype: IgG2aκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001157531.1
• UniProt accession no.: P18337
• compatibility: for use with ColorWheel^® Dyes (Required, sold separately)
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Sell(20343)

Description

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone MEL-14 is a rat monoclonal antibody that detects L-Selectin (CD62L).

Immunogen

C3H/eb mouse B lymphoma 38C-13 cells

Application

Quality Control Testing:

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis (FC): Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1061, Anti-Mouse CD62L (MEL-14) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of a PE-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

L-selectin (UniProt: P18337; also known as CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, Lymphocyte antigen 22, Ly-22, Lymphocyte surface MEL-14 antigen, CD62L) is encoded by the Sell (also known as Lnhr, Ly-22, Ly22) gene (Gene ID: 20343) in murine species. CD62L is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-28) and a propeptide (aa 29-38), which are subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 39-332), a transmembrane domain (aa 333-355), and a cytoplasmic domain (aa 356-372). CD62L is a calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. It is predominantly expressed in lymphoid tissue and mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. It is also reported to promote initial tethering and rolling of leukocytes in endothelia. Interaction with selectin P ligand (SELPLG)/P-selectin glycoprotein ligand-1 (PSLG1) and podocalyxin-like protein 2 (PODXL2) is shown to be essential for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan modification of SELPLG and PODXL2, and tyrosine sulfation modifications of SELPLG. (Ref.: Ivetic, A., et al. (2019). Front. Immunol. 10; Article 1068).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable