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CMC0012

Sigma-Aldrich

XLDNA SIG10 Electrocompetent cells

for BAC & cosmid cloning

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

biological source

Escherichia coli

grade

for molecular biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA

Components

  • XLDNA SIG10 electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCJ6765
SLCJ5122
SLCF2775
SLCD3496
SLBV7414

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