CMC0007

SIG10 5a Chemically Competent Cells

Escherichia coli, rod shaped

• Synonym(s): Chemically Competent Cells
• UNSPSC Code: 41106202
• NACRES: NA.85

Properties

• product name: SIG10 5a Chemically Competent Cells, for DNA plasmid production
• biological source: Escherichia coli
• grade: for molecular biology
• form: buffered aqueous solution
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: chemically competent; transformation efficiency: ≥1 x 10⁸ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5α^™ and directly replace them in cloning protocols.
They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide solutions for a wide range of applications at economical prices.

Genotype

fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Features and Benefits

• Direct replacements for standard cloning strains like DH5α^™, DH10B, JM109, TOP10, XL1-Blue, etc.
• Optimized genetics for high yields: endonuclease and recombination minus
• Blue - white screening

Components

• SIG10 5a chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Legal Information

DH5α is a trademark of Invitrogen Corp.

Safety Information

• Storage Class: 10 - Combustible liquids