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Key Documents

LB7

98062312, human blood, Lymphoblastoid

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About This Item

UNSPSC Code:
41106514

product name

LB7, 98062312

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human lymphocytes, normal, EBV-transformed

Cell Line Description

Established by EBV transformation of mononuclear cells from the peripheral blood of a 51 year old male. T cells from the sample have been used as normal control for DNA repair studies and mutation analysis.

Application

Toxicity and hprt mutation studies

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5%CO2; 37°C. Cells form floating aggregates.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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