B1D6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 98042014-DNA-5UG; pkg of vial of cells 98042014-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: P3X63Ag8
• Reactivity: Anti-annexin II, human
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Not specified

Cell Line Description

The hybridoma B1D6 was obtained by immunising BALB/c mice with human placental extract followed by intravenous injections with purified Fc gamma-receptor (receptor for the Fc portion of immunoglobulins, FcR). Spleen cells were then fused with the myeloma P3X63Ag8 to establish B1D6 secreting IgG1, kappa, monoclonal antibodies. This antibody has been shown to stain FcR-positive areas in sections of placental tissues, inhibited the haemadsorption of placental tissue of erythrocytes sensitised with IgG antibodies and the agglutination of these erythrocytes by FcR.

Immunogen

Human placenta FcR

Application

FcR studies

Culture Medium

RPMI 1640 + 2 mM glutamine + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.