VPM 45
93113034, Reactivity: Anti-MHC class II DQβ chain, ovine
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About This Item
UNSPSC Code:
41106514
biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0
Reactivity
Anti-MHC class II DQβ chain, ovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS0 x Mouse BALB/c spleen
Cell Line Description
The antibody cross-reacts with bovine class II and reacts with MHC class II on cell surface and on immunoblots. It also reacts with a polymorphic determinant.
Immunogen
Sheep thymocytes
Application
Identifying and characterising sheep MHC class II molecules
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C. Saturation density is reached at 3-9x100,000 cells/ml.
Other Notes
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Certificates of Analysis (COA)
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