By immunoblotting against the reduced protein, the antibody reacts with bands at 85 kDa and 62 kDa and a series of further cleaved active forms. It also reacts with non-reduced calpain LP-85. The antibody does not cross-react with other calpain family members (calpain LP-82, μ-calpain, m-calpain, calpain-94, ncl-2, ncl-3, etc.).
Immunogen
synthetic peptide corresponding to a unique insert found in the ELF-hand (the calcium binding domain-IV in the large subunit) of rat LP-85 calpain (lens-specific calpain).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol and 15 mM sodium azide
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