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Sigma-Aldrich

AccuTaq™ LA 10× Buffer

10X Buffer for long and accurate PCR

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0.5 ML
$51.00

$51.00

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0.5 ML
$51.00

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UNSPSC Code:
41106306

$51.00

List price$60.00Save 15%
Web-Only Promotion

Available to ship onApril 10, 2025Details

grade

Molecular Biology
for molecular biology

Quality Level

200

form

liquid

packaging

vial of 0.5 mL

concentration

10 ×

technique(s)

PCR: suitable

storage temp.

−20°C

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General description

AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Application

AccuTaq™ LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.[1]
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum[2]
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)[3]

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000295519
0000295519
SLCP4419
SLCF8408
SLCB2592

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Karen Dawson et al.
PloS one, 5(12), e15204-e15204 (2010-12-15)
Genetic variation is the driving force of evolution and as such is of central interest for biologists. However, inadequate discrimination of errors from true genetic variation could lead to incorrect estimates of gene copy number, population genetic parameters, phylogenetic relationships
Yolande Bertin et al.
Applied and environmental microbiology, 70(1), 61-68 (2004-01-09)
Of 220 Shiga toxin-producing Escherichia coli (STEC) strains collected in central France from healthy cattle, food samples, and asymptomatic children, 12 possessed the eae gene included in the locus of enterocyte effacement (LEE) pathogenicity island. Based on gene typing, we
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA
Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
Vandana Jaiswal et al.
PloS one, 10(6), e0129400-e0129400 (2015-06-16)
TaGW2 is an orthologue of rice gene OsGW2, which encodes E3 RING ubiquitin ligase and controls the grain size in rice. In wheat, three copies of TaGW2 have been identified and mapped on wheat homoeologous group 6 viz. TaGW2-6A, TaGW2-6B
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Protocols

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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