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A8549

Sigma-Aldrich

Albumin, biotin labeled bovine

lyophilized powder

Synonym(s):

Biotin labeled albumin

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
34058011
NACRES:
NA.25

biological source

bovine

Quality Level

assay

≥80% protein basis (biuret)

form

lyophilized powder

extent of labeling

8-16 mol biotin per mol albumin

technique(s)

bioconjugation: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

application

Biotin labeled bovine albumin has been used as a component in myosin V processivity experiments. It has also been used to provide biotin tags for neutravidin coating and also to suppress nonspecific cell adhesion during preparation of tension gauge tether (TGT) surface.
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues. In spleen, biotin-BSA binds to red pulp sinuses, but not white pulp vessels (including the central arteriole) nor the marginal sinus. In the thymus, prominent binding was restricted to the capillaries of the corticomedullary area.

Physical form

Contains sodium citrate buffer salts

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Integrin Molecular Tension within Motile Focal Adhesions
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We use photothermal microscopy to detect and image individual gold nanoparticles that are either embedded in a polymer film or immobilized in an aqueous environment. Reducing the numerical aperture of the detection optics allows us to achieve a 200-fold-enlarged detection
Tilting and Wobble of Myosin V by High-Speed Single-Molecule Polarized Fluorescence Microscopy
Beausang JF, et al.
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Taikopaul Kaneko et al.
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Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin.
Maximilian T Strauss et al.
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Self-assembled DNA nanostructures feature an unprecedented addressability with sub-nanometer precision and accuracy. This addressability relies on the ability to attach functional entities to single DNA strands in these structures. The efficiency of this attachment depends on two factors: incorporation of

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