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A6680

N-Acetylneuraminic Acid Aldolase from microorganisms

lyophilized powder, ≥20 units/mg protein (biuret)

Synonym(s):

N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase

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Size/SKUAvailabilityPrice
25 units
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$1,120.00

About This Item

CAS Number:
NACRES:
NA.32
UNSPSC Code:
12352204
Beilstein/REAXYS Number:
2697172
MDL number:
EC Number:
Specific activity:
≥20 units/mg protein (biuret)

$1,120.00


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form

lyophilized powder

Quality Segment

specific activity

≥20 units/mg protein (biuret)

mol wt

~98 kDa

storage temp.

−20°C

General description

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q

Physical form

Lyophilized powder containing mannitol and EDTA

Other Notes

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

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A8811H6512C8271
description

lyophilized powder, ≥20 units/mg protein (biuret)

description

ammonium sulfate suspension, 10-20 units/mg protein

description

Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100 units/mg protein (enzyme + BSA)

description

≥85% (HPLC)

specific activity

≥20 units/mg protein (biuret)

specific activity

10-20 units/mg protein

specific activity

≥100 units/mg protein (enzyme + BSA)

specific activity

-

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

~98 kDa

mol wt

-

mol wt

84.1 kDa

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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